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RUCDR maintains
secure, state-of-the-art facilities with redundant back-up
systems, including natural gas and diesel powered emergency
electric generators.
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Each operation is
computerized to minimize sample mislabeling and/or
cross-contamination.
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A small aliquot of
blood is stored from which DNA can be extracted at a later date
to resolve any sample identity, sample contamination, or
pedigree relationship issues. DNA comparisons are done using
multiplex PCR of highly polymorphic microsatellite loci.
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Testing for
mycoplasma by PCR and two independent cell-culture procedures.
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All lymphoblastoid
cell lines (LCLs) are free of mycoplasma, bacterial, and fungal
contamination.
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All cryopreserved
LCLs are viability tested.
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Cryopreserved LCLs
are stored in multiple locations, including a remote backup
site, with around the clock monitoring.
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Genomic DNA is
extracted using state-of-the-art automated extraction systems.
DNA quality is verified using restriction enzyme digestions and
agarose gel electrophoresis, PCR, and by UV spectroscopy.
Excellent results in hundreds of thousands of genotype assays
are a testimonial to the quality of RUCDR DNA.
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New kits, reagents,
and protocols are validated before routine use.
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Continuing education,
training, and safety programs are provided to all RUCDR
personnel.
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Real-time proprietary
databases and advanced barcode technology are used for tracking
samples, with hard-wired and wireless communication to mainframe
computers.
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Timely reports and
schedules are provided to clients and funding agencies.
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Senior RUCDR
personnel are on call for rapid response to equipment or
facility alarm conditions.
